Characterization of Accdeaminase in Plant 361 Characterization of Acc Deaminase in Plant Growth Promoting Pseudomonas from Tannery Sludge

The plant growth promoting strains isolated from tannery sludge and charaeteri/ed is Pseudomonas strain. The plant growth promoting Pseudomonas pittidu can utilize 1-aniinocyclopropanc-l-carboxylase (ACC) as a sole nitrogen source because it possess the unusual enzyme ACC dcaminase, which hydroly/es ACC to ammonia and a-ketobutyrate. This enzyme which is though to be intimately involved in the mechanism that the bacterium uses to promote root elongation in developing seedlings, under field study was purified and characterized. ACC deaminase activity is found in the cytoplasm of the bacterium, is induced by low levels (l00nM) of ACC, and has a temperature optimum at approximately 30’c and a pll optimum of 8.5. These properties are very similar to those reported for ACC” deaminase from another soil bacterium Pseudomonas strain. In heavily contaminated soil, sludge where the pollutant exceeds the limit of plant tolerance, it may be possible to treat plant with plant growth promoting bacteria, increasing plant growth on polluted soils.